Evaluation of Sustained Release Matrix Tablets of Cilostazol

Evaluation of Sustained Release Matrix lozenges of CilostazolDevelopment and in Vitro-in Vivo Evaluation of Sustained Release Matrix tabularizets of CilostazolKeywordsCilostazol Pharmacokinetics ER Matrix plankt In Vitro KineticsABSTRACTThe objective of this research had to manufacture extend dissolve ground substance tablet of Cilostazol and to evaluate its in vitro medicate turf out and in vivo absorption. The dosage form was designed by selection of miscellaneous polymers such as Hypromellose, Kollidon SR, Xanthan gum, Ethyl cellulose, Eudragit Polymers. Microcryst e veryine cellulose and lactose as dilutents to ramp up matrix tablets and povidone as granulating binders. The tablets were inclined(p) by Direct compression, wet granulation and Melt extrusion techniques. Optimized preparedness of Cilostazol matrix tablets was prepared by use 7% HPMC K100M polymer, 39 % MCC, 3% of povidone as binder. Matrix tablets were compressed with optimized forgive f pitifuling gr anules of uniform medicate confine. This in vitro do medicates firing off showed the panoptic the release period up to as per desired specifications. The matrix make by HPMC, MCC and Povidone had been showed satisfactorily with the controlled resistance. Bioavilability study of this wet granulation dosage statutetion in rabbit model showed 24 h preserve drug release in vivo. A correlation (R2= 0.9833) was founded in the midst of the in vitro drug release and the in vivo drug absorption. The results suggested that wet granulation with is a sufficient method to civilize a continue release Cilostazol and it groundwork be Performed therapeutically better than conventional IR dosage form.1. IntroductionIn this study the Cilostazol sustained release matrix tablet was developed with dissimilar polymers. Since the IR dosage form produces and locating effect head of bear due to drug oscillation in plasma. The challenge become to develop a matrix tablets are due to drug morp hology and highly insoluble in nature . In the present study, a sustained release dosage form of Cilostazol has been developed that enables less frequent ad ministering of drug . Matrix tablets of Cilostazol were formed by appropriate combination of HPMC and Povidone and lactose monohydrate,MCC and Kollidon K30 was chosen for matrix tablet to extend duration of drug release.Cilostazol and its metabolites are keep down the platelet aggregation and exert vasodilatory action by inhibiting phosphodiesterase activity and encamp degradation with a resultant increase in cAMP in platelets and blood vesselsThe objectives of research were 1) To analyze the physical and chemical characters of prepared Tablets 2) To discharge the effect of polymers and to study the release kinetics, 3) in-vivo study for the stable formula.2. Materials and Methods2.1. MaterialsCilostazol was obtained from IPCA lab, Mumbai, India. Hypermellose (Methocel K100M CR), Povidone K30 received as a gift sample from C olorcon Pvt Ltd. Kollidon K30 was obtained from BASF. All other Reagents were purchased from local suppliers, India and were of analytic grade.2.2. Drug and Excipient InteractionsDrug Excipient interaction study was investigated by DSC ( divers(prenominal)ial scanning calorimeter). The DSC Thermo gigabyte of only drug and Drug+Excipient mixtures were noted. The samples were separately jammed in atomic number 13 cells and kept a set in Metler TA 4000 Thermal analyzer.2.3. conceptualization1. Dispensing All the ingredients were dispensed accurately as per formula meter.2. Sifting metrical quantity (refer table no.4.5) of Cilostazol, Microcrystalinecellulose (Avicel PH-101), HPMC K100M, were passed through 30, Microcrystalinecellulose(PH-102), Aerosil-200, through 40, yellow(a) oxide of iron and Magnesium stearate were passed through 60 .3. Mixing Measured quantity (refer table no.4.5) of Cilostazol, Microcrystalinecellulose (Avicel PH-101), were interracial in polybag for 15 min, to it added yellow oxide of iron and mixed for 5 min. in RMG at 2.4 RPM .4. Preparation of Binder event Measured quantity (refer table no.4.5) of IPA and Water were poured in stainless trade name beaker, to it added PVPK-30 with stirring continuously by glass rod till turn completely and clear solution is obtained.5. Wet Granulation Granules were prepared by wet granulation method in RMG at 2.4 RPM for 15 min, using step 4 binder solution. Prepared granules dried at 60 c till LOD reaches less than 2.5% and finally sifted through 30.6. Mixing Mixed the thrifty quantity (refer table no.4.5) of Microcrystaline cellulose (Avicel PH-102), HPMC K100M, Aerosil-200, in polybag for 15 min with granules obtained in step 5.7. Lubrication Above granules are lubricated with measured quantity of magnesium stearateIn the trial 5 tautness of HPMC K100M is cut down from 10% to 8% and trials 6,7,8 from 10% to 7% .2.4. Physical Characterization of TabletsThe prepared tablets were subjected to various physical characterization studies. Weight variation test was performed with 20 tablets with an electronic balance. Tablets hardness was determined using Monsanto (Standard type) tablet hardness tester. thickness was measured by a venier caliper (Mitutoyo, Japan). Friability was calculated using a Roche friabilator (Basel, Switzerland)2.5. Drug Content of Tablets (Assay by HPLC)Cilostazol USPChromatographic ConditionsThe drug content of the formulated tablets was estimated by HPLC method. Column Stainless steel column packed with octadecylsilane silica gel for chromatography ,c18 ,1504.6 mm,5m(Inertsil ODS-3 is suitable) Mobile phaseAcetonitrileMthanolWater(7310by volume), filter and degas. pay heed rate 1.0ml / min Wavelength 254nm Diluent Methanol Injection Volume 10l Temperature 270C 10C Retention time Cilostazol- about 9.4 minutes. Cilostazol was analyzed by HPLC at a wavelength of 254 nm.2.6. In Vitro dissolution StudiesIn-Vitro dissolution Studies (Dissolution an alysis by HPLC)Dissolution testing for the amount of drug-substances released was studied using the following dissolution parametersTable Dissolution parameters and specifications for CilostazolAcceptance criteria As given table no.4.15Dissolution Parameters medium 900 ml, 0.3% SLS in 6.8 Phophate bufferApparatus USP Apparatus 2Paddle Speed 75 RPM Temperature370C 0.50c. time1,4,8,12,24 hours Chromatographic Conditions Instrument HPLC(Hitachi) Column Stainless steel column packed with octadecylsilane silica gel for chromatography, C-18, 150cm 4.6mm, 5m (Inertsil ODS-3 is suitable) Mobile Phase AcetonitrileMthanolWater(7310 by volume) filter and degas. Flow rate 1.0 ml/min Wavelength 254nm Injection Volume 20l Diluent Methanol, Dissolution ordinary Temperature 270C 10C. The release studies were conducted in duplicate. Mean % cumulative drug release was plan against time (hours).2.7. Drug release Kinetics and MechanismKinetics of drug release was determined by fitting data toTa ble 1. physical composition of extended release matrix tablet of Cilostazol different models such as zero order (M = kt), first order equation (M = lnM0+ kt), Higuchi model (M = kt) and KorsemeyerPeppas equation (M = ktn). The value of n = 0.5 denotes movement I dissemination (Fickian), 0.5 n = 1, for case II transport and n 1 for super case II transport. Where M is the amount of drug (%) released after time t Where M0is the amount of drug released at (0) zero time k is the release rate constant, n is the exponent. Drug release following particular machine is judged by the linearity of plot2.8. Stability StudiesStability studies were conducted on SR Tablets of select batch to assess their stability with respect to their physical appearance, drug content and release characteristics after storing at 25C under 60% relative humidity (RH) and 40C under 75% RH for 6 months 8.2.9. Pharmacokinetic EvaluationThe animal studies were performed as per guidelines for the Care and utilizat ion of Animals that were approved by the Animal Ethics Committee. Male rabbits (Albino) with average incubus of 2.5 kg were housed in standard cage individual, which well ventilated with air, humidity and temperature control.50 mg equivalent weight weight of Cilostazol sustained Release Tablets with and 50mg equivalent weight of Cilostazol 50mg IR tablet was administered to 2 groups viva vocely (N = 4) via silicone golosh gastric canulization tube.A wooden rod was kept between the jaws of rabbits mouth. A gastric tube was centrally position over the hole in mouth (21.22). With the help of gastric intubation tube the tablets were administered in to the stomach of rabbit by set on the tip in it. After administered the spoken dose, 5 ml of wet was given to facilitate the admittance of the tablets. Rabbits were kept fasting over shadow but access to water ad libitum In a heparin zed branule (G22, G24) 2 ml of blood samples were collected, which placed in the fringy ear vein , a t each of the pre determined times i.e., 0.25 Hr, 0.5 Hr, 1 Hr, 2 Hr, 4 Hr, 6 Hr, 8 Hr and 24 Hr after governingSamples were transferred to eppendrof centrifuge tube and centrifuged at 3000 rpm for 10 min. The separated organic layer pass on be collected and melt to dryness under a gentle steam of nitrogen gas. The obtained residues leave be reconstituted in organic solvent with vortex mixing, from which aliquot will be injected to HPLC system.supernatant plasma was separated and transferred and stored at 20C until Analyzed.in to 96 well plate2.10. In Vivo entropy analytic thinkingThe maximum plasma concentration (Cmax) and the time to reach the maximum concentration (tmax) were directly obtained from the observed values. The area under the curve up to 24 h after administration (AUC) was calculated by the trapezoidal territorial dominion from the observed values.3. Results and DiscussionIn this study, the matrix tablets were prepred using various types of polymers and differen t composition. of polymers (Table 1) of matrix forming polymers (HPMC, sodium CMC and MCC) with the help of granulating agent, PVP was used as Binder. In vitro studies conducted for all the faces. Extended release of drug was in the order of CW1 Figure 1). charge per unit of drug release was significant (p Figure 1, CW5). It seems the chemical mechanism is by diffusion method. Physical characteristics of matrix tablets were shown in Table 2.There was no whatever significant burst effect in the optimized HPMC matrix tablets that showed a low possibility of dose dumping and avoids toxicity (in vivo). The Release kinetics of matrix tablets was determined by fitting the drug release data in different established models they are zero order, first order, Higuchi model, Korsemeyer-Peppas equation.Table 3shows values of backsliding coefficient, release constant and exponent n. First order release data was not satisfactory. The data suggested that kinetics of drug release of DVF5 was be st explained by Korsemeyer-Peppas equation (R2= 0.991, n = 0.60). This indicated combined effect of diffusion and erosion mechanism on the release of drug.The stability results of storing at 25C/60% RH and 40C/75% RH for 6 months as per ICH guidelines evidenced any change in physical parameters and appearance and very slight change in dissolution pattern. Based on the functional stability data 2 years shelf life can be provided.Figure 1. In vitro release profile of Cilostazol SR tablets.Table 2. Drug release kinetic of Cilostazol SR tablet faces.Next, the stable formula were designated for its in vivo test in rabbit. Plasma concentration and pharmacokinetic parameters after oral administration of formulated ER matrix tablet CW5 and Cilostazol IR tablets 50mg were summarized inFigure 2andTable 3. No sustained blood aim was observed after oral administration of the IR formulation. The formulated matrix Tablet (CW5) showed significant lowerCmaxthan the IR formulation (P max(tmaxis 6 hr) as compared with immediate release formulation (tmaxis 0.55 hr). The AUC increased from 11190.30 hr*ng/ml to 295396.49 hr*ng/ml for ER tablets. value of Cmaxand tmaxclearly indicated that the drug release was sustained to about 24 hours after oral administration in rabbits (n = 4). CW5 Tablets maintained prolonged plasma concentration up to about 24 hours. The sustained plasma concentration of new formulation (CW5) indicates its extended drug release in vivo absorption.The Results demonstrated that the hydrophilic polymers were successfully utilized for formulating Cilostazol extended release matrix tablets. By wet granulate with povidone . Moreover the extended release matrix tablets have a uncomparable advantage of lessening chance of dose dumping and to avoid side effects. The investigated extended release matrix tablets were adequate to maintaining constant plasma level of Cilostazol up to 24 hours in rabbits.Figure 2. Profile shows mean plasma concentration of Cilostazol against time, following oral administration of IR tablets and SR Tablets (CW5) to rabbits. Data are represented as mean SD (n = 4).Table 3. Mean (SD) pharmacokinetic parameters of Cilostazol in Rabbits (n = 4) orally administered with IR tablets and ER tablets CW5 (50 mg).4. ConclusionA new sustained release formulation of Cilostazol has been developed for its in-vitro drug release and in-vivo absorption. Extended release matrix tablet were found to be an effective to maintain the drug level in plasma. Bioavailability studies can be carried out to assess the gain of this formulationand in comparison with existing IR products in the market formulations on healthy human volunteers.